Journal: Animal Microbiome

Article Title: High throughput in vitro characterization of pectins for pig(let) nutrition

doi: 10.1186/s42523-021-00129-w

Figure Lengend Snippet: The effect of different substrates on in vitro simulated piglet gut microbiota as determined by 16S rRNA gene (V3-region) amplicon sequencing (Weighted Unifrac distance metrics). All fermentations were carried out in quadruplicate (with exceptions as stated in Fig. ). a Polysaccharides sourced from pectins with inulin and AG (arabinogalactan) as prebiotic controls. b Sugar beet pectins with different degrees and patterns of esterification. c Different pectin polysaccharides at additive dose of 1% and 0.5%. d Oligossaccharides derived from pectins with FOS (fructooligosaccharides) and GOS (galactooligosaccharides) as prebiotic controls. See Table substrate abbreviations and details on degree of esterification and acetylation for the different substrates

Article Snippet: The microbiota composition of in vitro fermentation samples was profiled using tag-encoded V3-region 16S rRNA gene NextSeq-based (Illumina, CA, USA) high throughput sequencing as previously described [ ].

Techniques: In Vitro, Amplification, Sequencing, Derivative Assay

Journal: Animal Microbiome

Article Title: High throughput in vitro characterization of pectins for pig(let) nutrition

doi: 10.1186/s42523-021-00129-w

Figure Lengend Snippet: Piglet gut microbiota composition (summarized on species level) as determined by 16S rRNA gene (V3-region) amplicon sequencing after in vitro simulated colon fermentation of different substrates as indicated. Only taxa with average relative abundance > 1% shown. All fermentations were carried out in quadruplicate unless otherwise stated. The additive dose is 1% except for groups with specific description of using 0.5% additive dose. Media-GM control, no added pectin saccharides, FOS fructooligosaccharides derived from chicory (n = 3), GOS galactooligosaccharides derived from milk, Inulin inulin derived from chicory (n = 3), AG arabinogalactan, PP pea pectin (n = 2), SBPulp pulp from sugar beet (n = 2), ModSBP modified sugar beet pectin, SBP sugar beet pectin, SBP_0.5% same, but 0.5% substrate; SBP_RG-I (n = 3) and SBP-RG-I_0.5 (n = 2), sugar beet pectin derived rhamnogalacturonan I, C-HMP and C-HMP_0.5, citrus derived high methoxyl pectin (n = 3) 1% substrate, C- PG citrus derived polygalacturonic acid (constituting pectins through methyl esterification), C-RG-I citrus derived rhamnogalacturonan I, C-RG-I_0.5% LM1B, low methoxyl pectin, random esterification pattern, LM1C low methoxyl pectin, random esterification pattern, LM1D low methoxyl pectin, random esterification pattern, LM2 low methoxyl pectin, blocky esterification pattern, LM2A low methoxyl pectin, blocky esterification pattern (n = 3), Ar arabinans derived from sugar beet pectin, DebAr debranched arabinans derived from sugar beet pectin (n = 3), ModAr modified arabinans, MixArDebArPG 1:1:1 substrate mixture of polygalacturonic acid, arabinans and debranched arabinans. See Table for details on degree of esterification and acetylation for the different substrates

Article Snippet: The microbiota composition of in vitro fermentation samples was profiled using tag-encoded V3-region 16S rRNA gene NextSeq-based (Illumina, CA, USA) high throughput sequencing as previously described [ ].

Techniques: Amplification, Sequencing, In Vitro, Derivative Assay, Modification

Journal: Animal Microbiome

Article Title: High throughput in vitro characterization of pectins for pig(let) nutrition

doi: 10.1186/s42523-021-00129-w

Figure Lengend Snippet: The effect of substrate on Shannon diversity index of in vitro simulated piglet gut microbiota as determined by 16S rRNA gene (V3-region) amplicon sequencing. The additive dose is 1% except for groups with specific description of using 0.5% dose. The data shown are averages of 4 replicates, unless otherwise stated. a Polysaccharides sourced from pectins with inulin (n = 3) and AG (arabinogalactan) as prebiotic controls. PP pea pectin (n = 2), SBPulp pulp from sugar beet (n = 2), ModSBP modified sugar beet pectin, SBP sugar beet pectin, SBP_RG-I sugar beet pectin derived rhamnogalacturonan I (n = 3), C-HMP citrus derived high methoxyl pectin(n = 3 for 1% dose), C-PG citrus-derived polygalacturonic acid (constituting pectins through methyl esterification), C-RG-I citrus derived rhamnogalacturonan I; b sugar beet pectins with different degrees and patterns of esterification. SBP sugar beet pectin, LM1B low methoxyl pectin, random esterification pattern, LM1C low methoxyl pectin, random esterification pattern, LM1D low methoxyl pectin, random esterification pattern, LM2 low methoxyl pectin, blocky esterification pattern, LM2A low methoxyl pectin, blocky esterification pattern (n = 3); c different pectin polysaccharides at additive dose of 1% and 0.5%. d Oligossaccharides derived from pectins with FOS (fructooligosaccharides, n = 3) and GOS (galactooligosaccharides) as prebiotic controls. AR arabinans derived from sugar beet pectin (n = 3), DebAr debranched arabinans derived from sugar beet pectin (n = 3), ModAr modified arabinans, MixArDebArPG 1:1:1 substrate mixture of polygalacturonic acid, arabinans and debranched arabinans. In all cases compared against FOS, GOS and inulin (n = 3). *Significantly different from FOS; #significantly different from GOS and ¤significantly different from inulin; ^significantly different from Media-GM control, the significant marks are assigned if P values < 0.05. See Table for details on degree of esterification and acetylation for the different substrates

Article Snippet: The microbiota composition of in vitro fermentation samples was profiled using tag-encoded V3-region 16S rRNA gene NextSeq-based (Illumina, CA, USA) high throughput sequencing as previously described [ ].

Techniques: In Vitro, Amplification, Sequencing, Modification, Derivative Assay, IF-P